Sop and calibration of uv
1.0 OBJECTIVE To describe the procedure for Calibration of UV-Vis. Spectrophotometer. 2.0 SCOPE This SOP is applicable for Calibration of UV-Vis. Spectrophotometer. 3.0 RESPONSIBILITY Officer/ Executive - Quality Control. 4.0 ACCOUNTABILITY Manager - Quality Control. 5.0 PROCEDURE 5.1 Calibration 5.1.1 Calibration of UV-VIS Spectrophotometer is done in four steps. A ] Control of Wavelength B] Control of Absorbance C] Limit of Stray Light D] Resolution Power 5.1.2 Operate the instruments as per SOP. 5.2 Control of Wavelength 5.2.1 Weight accurately 1.0 gm of Holmium Oxide and dissolve it in 1.4 M Perchloric acid solution. Makeup to 25 ml with the same solvent. 5.2.2 Select the method file of Control of Wavelength in the instrument. 5.2.3 After selecting the file press Reference button for baseline correction. 5.2.4 Then fill the Cuvette with 1.4M Perchloric acid and put in the sample cubicle and press reference to zero. 5.2.5 After auto ze...